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Western blot analysis of MFAP5 antibody in K562 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human Skeletal muscle reacted with MFAP5 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of MFAP5 using rabbit polyclonal MFAP5 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MFAP5 gene.
Western blot analysis of MFAP5 antibody in K562 cell line lysates (35ug/lane)

MFAP5 Antibody

CATALOG NUMBER: 62-819

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen:
This MFAP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 133-160 amino acids from the C-terminal region of human MFAP5.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
20 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MFAP5
Additional Names:
Microfibrillar-associated protein 5, MFAP-5, MP25, Microfibril-associated glycoprotein 2, MAGP-2, MFAP5, MAGP2
Protein Accession Number:
Q13361
Protein Gi Number:
2498553
Ncbi Gene Id Number:
8076
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MFAP5 is a 25-kD microfibril-associated glycoprotein which is rich in serine and threonine residues. The protein lacks a hydrophobic carboxyl terminus and proline-, glutamine-, and tyrosine-rich regions, which are characteristics of a related 31-kDa microfibril-associated glycoprotein (MFAP2). The close similarity between these two proteins is confined to a central region of 60 aa where precise alignment of 7 cysteine residues occurs. The structural differences suggest that this protein has some functions that are distinct from those of MFAP2.
Background Reference 1:
Albig,A.R., Becenti,D.J. Microvasc. Res. 76 (1), 7-14 (2008)
Background Reference 2:
Miyamoto,A., Lau,R. J. Biol. Chem. 281 (15), 10089-10097 (2006)
Background Reference 3:
Penner,A.S., Rock,M.J. J. Biol. Chem. 277 (38), 35044-35049 (2002)
Background Reference 4:
Hatzinikolas,G. J. Biol. Chem. 273 (45), 29309-29314 (1998)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-819

List Size:
400 ul

List Price:

$495.00

Datasheet
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