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Western blot analysis of BTBD1 antibody in 293 cell line lysates (35ug/lane)
Western blot analysis of BTBD1 antibody in mouse cerebellum tissue lysates (35ug/lane)
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of BTBD1 antibody in 293 cell line lysates (35ug/lane)

BTBD1 Antibody

CATALOG NUMBER: 62-822

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This BTBD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 295-322 amino acids from the Central region of human BTBD1.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
53 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BTBD1
Additional Names:
BTB/POZ domain-containing protein 1, Hepatitis C virus NS5A-transactivated protein 8, HCV NS5A-transactivated protein 8, BTBD1, C15orf1, NS5ATP8
Protein Accession Number:
Q9H0C5
Protein Gi Number:
20137477
Ncbi Gene Id Number:
53339
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
BTBD1 binds topoisomerase I. The N-terminus contains a proline-rich region and a BTB/POZ domain (broad-complex, Tramtrack and bric a brac/Pox virus and Zinc finger), both of which are typically involved in protein-protein interactions. Subcellularly, the protein localizes to cytoplasmic bodies.
Background Reference 1:
Pisani,D.F., Cabane,C. Cell Death Differ. 11 (11), 1157-1165 (2004)
Background Reference 2:
Xu,L., Yang,L. Exp. Cell Res. 288 (1), 84-93 (2003)
Background Reference 3:
Xu,L., Yang,L. BMC Genomics(1),(2002)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-822

List Size:
400 ul

List Price:

$495.00

Datasheet
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