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Western blot analysis of HSPA1A using rabbit polyclonal HSPA1A Antibody (S41) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HSPA1A gene (Lane 2).
Western blot analysis of HSPA1A using rabbit polyclonal HSPA1A Antibody (S41) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HSPA1A gene (Lane 2).

HSPA1A/HSPA1B Antibody

CATALOG NUMBER: 63-005

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Pig, Monkey, Rat, Chicken, Xenopus, Yeast, C. Elegans, Hamster, Zebrafish, Drosophila
Immunogen:
This HSPA1A/HSPA1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-48 amino acids from human HSPA1A/HSPA1B.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
70 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HSPA1A
Additional Names:
Heat shock 70 kDa protein 1A/1B, Heat shock 70 kDa protein 1/2, HSP70-1/HSP70-2, HSP701/HSP702, HSPA1A, HSPA1, HSX70
Protein Accession Number:
P08107
Protein Gi Number:
147744565
Ncbi Gene Id Number:
33033304
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
HSPA1A is a member of the heat shock protein 70 family. In conjunction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1.
Background Reference 1:
Mueller, T., et al., Transplantation 78(2):292-295 (2004).
Background Reference 2:
Fekete, A., et al., Pediatr. Res. 54(4):452-455 (2003).
Background Reference 3:
Broquet, A.H., et al., J. Biol. Chem. 278(24):21601-21606 (2003).
Background Reference 4:
Bruce, C.R., et al., Diabetes 52(9):2338-2345 (2003).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-005

List Size:
400 ul

List Price:

$519.00

Datasheet
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