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Fluorescent image of Hela cell stained with TEC Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TEC primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).TEC immunoreactivity is localized to Cytoplasm significantly.
Fluorescent image of Hela cell stained with TEC Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TEC primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).TEC immunoreactivity is localized to Cytoplasm significantly.
Western blot analysis in NCI-H292 cell line lysates (35ug/lane).
Western blot analysis of anti-TEC Pab in mouse liver tissue lysate
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Fluorescent image of Hela cell stained with TEC Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TEC primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).TEC immunoreactivity is localized to Cytoplasm significantly.

TEC Antibody

CATALOG NUMBER: 63-168

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For IF starting dilution is: 1:10~50
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
74 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TEC
Additional Names:
Tyrosine-protein kinase Tec, TEC, PSCTK4
Protein Accession Number:
P42680
Protein Gi Number:
158518392
Ncbi Gene Id Number:
7006
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TEC belongs to the TEC subfamily of non-receptor protein-tyrosine kinases containing a pleckstrin homology domain. TEC family kinases are involved in the intracellular signaling mechanisms of cytokine receptors, lymphocyte surface antigens, heterotrimeric G-protein coupled receptors, and integrin molecules. They are also key players in the regulation of the immune functions. TEC kinase is an integral component of T cell signaling and has a distinct role in T cell activation. TEC may be associated with myelodysplastic syndrome.
Background Reference 1:
Nore, B.F., et al., Biochim. Biophys. Acta 1645(2):123-132 (2003).
Background Reference 2:
Maltais, A., et al., Cancer Lett. 183(1):87-94 (2002).
Background Reference 3:
Lachance, G., et al., J. Biol. Chem. 277(24):21537-21541 (2002).
Background Reference 4:
Yang, W.C., et al., Immunity 12(4):373-382 (2000).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-168

List Size:
400 ul

List Price:

$495.00

Datasheet
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