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Western Blot at 1:1000 dilution Lane 1: A549 whole cell lysate Lane 2: NCI-H460 whole cell lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CTGF antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western Blot at 1:1000 dilution Lane 1: A549 whole cell lysate Lane 2: NCI-H460 whole cell lysate Lysates/proteins at 20 ug per lane.

CTGF Antibody

CATALOG NUMBER: 63-197

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat, Pig, Bovine
Immunogen:
This CTGF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 315-349 amino acids from the C-terminal region of human CTGF.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
38 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CTGF
Additional Names:
Connective tissue growth factor, CCN family member 2, Hypertrophic chondrocyte-specific protein 24, Insulin-like growth factor-binding protein 8, IBP-8, IGF-binding protein 8, IGFBP-8, CTGF, CCN2, HCS24, IGFBP8
Protein Accession Number:
P29279
Protein Gi Number:
116241320
Ncbi Gene Id Number:
1490
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Connective tissue growth factor (CTGF), a member of the CCN family of secreted matricellular proteins, regulates fibrosis, angiogenesis, cell proliferation, apoptosis, tumor growth, and metastasis.
Background Reference 1:
Li,M.H., Mol. Cancer Res.(10), 1649-1656 (2008)
Background Reference 2:
Bogatkevich,G.S., Am. J. Physiol. Lung Cell Mol. Physiol. 295 (4), L603-L611 (2008)
Background Reference 3:
Zhang,P., Tohoku J. Exp. Med. 215 (3), 199-206 (2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-197

List Size:
400 ul

List Price:

$495.00

Datasheet
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