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Western Blot at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western Blot at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.

ZAP70 Antibody

CATALOG NUMBER: 63-219

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ZAP70 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 252-282 amino acids from the Central region of human ZAP70.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
70 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ZAP70
Additional Names:
Tyrosine-protein kinase ZAP-70, 70 kDa zeta-chain associated protein, Syk-related tyrosine kinase, ZAP70, SRK
Protein Accession Number:
P43403
Protein Gi Number:
1177044
Ncbi Gene Id Number:
7535
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.
Background Reference 1:
Kuroyama, H., et al., Biochem. Biophys. Res. Commun. 315(4):935-941 (2004).
Background Reference 2:
Yang, Y., et al., Mol. Cell. Biol. 23(21):7667-7677 (2003).
Background Reference 3:
Kuhne, M.R., et al., J. Immunol. 171(2):860-866 (2003).
Background Reference 4:
Kremer, K.N., et al., J. Immunol. 171(1):360-367 (2003).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-219

List Size:
400 ul

List Price:

$495.00

Datasheet
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