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Western blot analysis of anti-SARS Antibody in NCI-H460 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with SARS antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of anti-SARS Antibody in NCI-H460 cell line lysates (35ug/lane)

SARS Antibody

CATALOG NUMBER: 63-225

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Hamster, Monkey, Mouse, Rat
Immunogen:
This SARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 394-422 amino acids from the C-terminal region of human SARS.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
59 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SARS
Additional Names:
Serine--tRNA ligase, cytoplasmic, Seryl-tRNA synthetase, SerRS, Seryl-tRNA(Ser/Sec) synthetase, SARS, SERS
Protein Accession Number:
P49591
Protein Gi Number:
19860217
Ncbi Gene Id Number:
6301
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Seryl-tRNA synthetase belongs to the class II amino-acyl tRNA family. This enzyme catalyzes the transfer of L-serine to tRNA (Ser) and is related to bacterial and yeast counterparts.
Background Reference 1:
Shimada,N., J. Biol. Chem. 276 (50), 46770-46778 (2001)
Background Reference 2:
Shah,Z.H., Hum. Mutat. 17 (5), 433-434 (2001)
Background Reference 3:
Heckl,M., FEBS Lett. 427 (3), 315-319 (1998)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-225

List Size:
400 ul

List Price:

$495.00

Datasheet
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