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Antibody is used in Western blot to detect BRD3 in mouse brain tissue lysate (Lane 1) and Saos-2 cell lysate (Lane 2).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Antibody is used in Western blot to detect BRD3 in mouse brain tissue lysate (Lane 1) and Saos-2 cell lysate (Lane 2).

BRD3 Antibody

CATALOG NUMBER: 63-366

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This BRD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 672-702 amino acids from the C-terminal region of human BRD3.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
80 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BRD3
Additional Names:
Bromodomain-containing protein 3, RING3-like protein, BRD3, KIAA0043, RING3L
Protein Accession Number:
Q15059
Protein Gi Number:
12643726
Ncbi Gene Id Number:
8019
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene was identified based on its homology to the gene encoding the RING3 protein, a serine/threonine kinase. The gene localizes to 9q34, a region which contains several major histocompatibility complex (MHC) genes. The function of the encoded protein is not known.
Background Reference 1:
Kaneko, H., et al.,Investig Allergol Clin Immunol 12(2):86-90 (2002).
Background Reference 2:
Thorpe, K.L., et al., Immunogenetics 44(5):391-396 (1996).
Background Reference 3:
Thorpe, K.L., et al., Gene 200 (1-2), 177-183 (1997).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-366

List Size:
400 ul

List Price:

$495.00

Datasheet
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