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Antibody staining AOC3 in human lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Western Blot at 1:2000 dilution Lane 1: human lung lysate Lane 2: human liver lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of AOC3 Antibody in CEM cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human colon carcinoma with AOC3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of CEM cells using AOC3 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Antibody staining AOC3 in human lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

AOC3 Antibody

CATALOG NUMBER: 63-509

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This AOC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 613-640 amino acids from the Central region of human AOC3.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For IHC-P starting dilution is: 1:25
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
85 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
AOC3
Additional Names:
Membrane primary amine oxidase, Copper amine oxidase, HPAO, Semicarbazide-sensitive amine oxidase, SSAO, Vascular adhesion protein 1, VAP-1, AOC3, VAP1
Protein Accession Number:
Q16853
Protein Gi Number:
2501336
Ncbi Gene Id Number:
8639
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Copper amine oxidases catalyze the oxidative conversion of amines to aldehydes in the presence of copper and quinone cofactor. The product is a major protein on the adipocyte plasma membrane. It has adhesive properties and also has functional monoamine oxidase activity.
Background Reference 1:
Lalor,P.F., et.al., J. Immunol. 169 (2), 983-992 (2002)
Background Reference 2:
Salmi,M., et.al., Am. J. Pathol. 161 (6), 2255-2262 (2002)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-509

List Size:
400 ul

List Price:

$495.00

Datasheet
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