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Western Blot at 1:4000 dilution Lane 1: K562 whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of ADH5 using rabbit polyclonal ADH5 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ADH5 gene.
Formalin-fixed and paraffin-embedded human hepatocarcinoma with ADH5 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of K562 cells using ADH5 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:4000 dilution Lane 1: K562 whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.

ADH5 Antibody

CATALOG NUMBER: 63-518

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rb
Immunogen:
This ADH5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 212-239 amino acids from the Central region of human ADH5.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
40 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ADH5
Additional Names:
Alcohol dehydrogenase class-3, Alcohol dehydrogenase 5, Alcohol dehydrogenase class chi chain, Alcohol dehydrogenase class-III, Glutathione-dependent formaldehyde dehydrogenase, FALDH, FDH, GSH-FDH, 111-, S-(hydroxymethyl)glutathione dehydrogenase, ADH5 (HGNC:253), ADHX, FDH
Protein Accession Number:
P11766
Protein Gi Number:
113408
Ncbi Gene Id Number:
128
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ADH5 is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This protein forms a homodimer. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis.
Background Reference 1:
Martins-de-Souza,D., et.al., Eur Arch Psychiatry Clin Neurosci 259 (3), 151-163 (2009)
Background Reference 2:
Iborra,F.J., et.al., J. Histochem. Cytochem. 40 (12), 1865-1878 (1992)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-518

List Size:
400 ul

List Price:

$495.00

Datasheet
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