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Western blot analysis of AGPAT3 Antibody in A2058 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with AGPAT3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of AGPAT3 Antibody in A2058 cell line lysates (35ug/lane)

AGPAT3 Antibody

CATALOG NUMBER: 63-587

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This AGPAT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 241-269 amino acids from the Central region of human AGPAT3.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
43 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
AGPAT3
Additional Names:
1-acyl-sn-glycerol-3-phosphate acyltransferase gamma, 1-acylglycerol-3-phosphate O-acyltransferase 3, 1-AGP acyltransferase 3, 1-AGPAT 3, Lysophosphatidic acid acyltransferase gamma, LPAAT-gamma, AGPAT3, LPAAT3
Protein Accession Number:
Q9NRZ7
Protein Gi Number:
12643817
Ncbi Gene Id Number:
56894
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
AGPAT3 is an acyltransferase that converts lysophosphatidic acid into phosphatidic acid, which is the second step in the de novo phospholipid biosynthetic pathway. This protein may be an integral membrane protein.
Background Reference 1:
Leung,D.W. et.al., Front. Biosci. 6, D944-D953 (2001)
Background Reference 2:
Lu,B., et.al., Biochem. J. 385 (PT 2), 469-477 (2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-587

List Size:
400 ul

List Price:

$495.00

Datasheet
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