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Western Blot at 1:1000 dilution + human brain lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:1000 dilution + human brain lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded mouse brain tissue reacted with ADCY8 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:1000 dilution + human brain lysate Lysates/proteins at 20 ug per lane.

ADCY8 Antibody

CATALOG NUMBER: 63-675

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This ADCY8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 946-972 amino acids from the Central region of human ADCY8.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
140 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ADCY8
Additional Names:
Adenylate cyclase type 8, ATP pyrophosphate-lyase 8, Adenylate cyclase type VIII, Adenylyl cyclase 8, AC8, Ca(2+)/calmodulin-activated adenylyl cyclase, ADCY8
Protein Accession Number:
P40145
Protein Gi Number:
729242
Ncbi Gene Id Number:
114
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ADCY8 is a membrane bound enzyme that catalyses the formation of cyclic AMP from ATP. The enzymatic activity is under the control of several hormones, and different polypeptides participate in the transduction of the signal from the receptor to the catalytic moiety. Stimulatory or inhibitory receptors (Rs and Ri) interact with G proteins (Gs and Gi) that exhibit GTPase activity and they modulate the activity of the catalytic subunit of the adenylyl cyclase provided by RefSeq].
Background Reference 1:
Martin,A.C.,et.al., Mol. Pharmacol. 75 (4), 830-842 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-675

List Size:
400 ul

List Price:

$495.00

Datasheet
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