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Western blot analysis of ADAMTS18 Antibody in mouse liver tissue lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with ADAMTS18 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the ADAMTS18 antibody detected the ADAMTS18 protein (arrow).
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of ADAMTS18 Antibody in mouse liver tissue lysates (35ug/lane)

ADAMTS18 Antibody

CATALOG NUMBER: 63-696

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This ADAMTS18 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1066-1095 amino acids from the C-terminal region of human ADAMTS18.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
135 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ADAMTS18
Additional Names:
A disintegrin and metalloproteinase with thrombospondin motifs 18, ADAM-TS 18, ADAM-TS18, ADAMTS-18, 3424-, ADAMTS18, ADAMTS21
Protein Accession Number:
Q8TE60
Protein Gi Number:
296439427
Ncbi Gene Id Number:
170692
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ADAMTS18 is a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. ADAMTS family members share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. This protein has a high sequence similarity to the protein encoded by gene ADAMTS16, another family member. It is thought to function as a tumor suppressor.
Background Reference 1:
Zeng,W., et.al., Biochim. Biophys. Acta 1760 (3), 517-524 (2006)
Background Reference 2:
Jin,H., et.al., Oncogene 26 (53), 7490-7498 (2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-696

List Size:
400 ul

List Price:

$495.00

Datasheet
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