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Western blot analysis of ALDH18A1 Antibody in K562 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with ALDH18A1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of ALDH18A1 Antibody in K562 cell line lysates (35ug/lane)

ALDH18A1 Antibody

CATALOG NUMBER: 63-710

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This ALDH18A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176-205 amino acids from the Central region of human ALDH18A1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
87 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ALDH18A1
Additional Names:
Delta-1-pyrroline-5-carboxylate synthase, P5CS, Aldehyde dehydrogenase family 18 member A1, Glutamate 5-kinase, GK, Gamma-glutamyl kinase, Gamma-glutamyl phosphate reductase, GPR, Glutamate-5-semialdehyde dehydrogenase, Glutamyl-gamma-semialdehyde dehydrogenase, ALDH18A1, GSAS, P5CS, PYCS
Protein Accession Number:
P54886
Protein Gi Number:
6226882
Ncbi Gene Id Number:
5832
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ALDH18A1 is a member of the aldehyde dehydrogenase family and encodes a bifunctional ATP- and NADPH-dependent mitochondrial enzyme with both gamma-glutamyl kinase and gamma-glutamyl phosphate reductase activities. The encoded protein catalyzes the reduction of glutamate to delta1-pyrroline-5-carboxylate, a critical step in the de novo biosynthesis of proline, ornithine and arginine.
Background Reference 1:
Hu,C.A., et.al., Amino Acids 35 (4), 665-672 (2008)
Background Reference 2:
Bicknell,L.S., et.al., Eur. J. Hum. Genet. 16 (10), 1176-1186 (2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-710

List Size:
400 ul

List Price:

$495.00

Datasheet
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