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Western blot analysis of SFRP1 Antibody in K562 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human testis tissue reacted with SFRP1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of SFRP1 Antibody in K562 cell line lysates (35ug/lane)

SFRP1 Antibody

CATALOG NUMBER: 63-754

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This SFRP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-55 amino acids from the N-terminal region of human SFRP1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
35 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SFRP1
Additional Names:
Secreted frizzled-related protein 1, FRP-1, sFRP-1, Secreted apoptosis-related protein 2, SARP-2, SFRP1, FRP, FRP1, SARP2
Protein Accession Number:
Q8N474
Protein Gi Number:
61216811
Ncbi Gene Id Number:
6422
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SFRP1 encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. Members of this family act as soluble modulators of Wnt signaling; epigenetic silencing of SFRP genes leads to deregulated activation of the Wnt-pathway which is associated with cancer.
Background Reference 1:
Huang,D., et.al., J. Cancer Res. Clin. Oncol. 136 (3), 395-401 (2010);
Background Reference 2:
Yang,Z.Q., et.al., Int. J. Cancer 125 (7), 1613-1621 (2009).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-754

List Size:
400 ul

List Price:

$495.00

Datasheet
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