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Western blot analysis of BPI Antibody in HL-60 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human tonsil tissue reacted with BPI Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of BPI Antibody in HL-60 cell line lysates (35ug/lane)

BPI Antibody

CATALOG NUMBER: 63-764

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This BPI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 293-324 amino acids from the Central region of human BPI.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
54 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BPI
Additional Names:
Bactericidal permeability-increasing protein, BPI, CAP 57, BPI
Protein Accession Number:
P17213
Protein Gi Number:
215274242
Ncbi Gene Id Number:
671
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
BPI encodes a lipopolysaccharide binding protein. It is associated with human neutrophil granules and has bactericidal activity on gram-negative organisms.
Background Reference 1:
Davila,S., et.al., Genes Immun. (2010) In press
Background Reference 2:
Guey,L.T., et.al., Eur. Urol. 57 (2), 283-292 (2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-764

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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