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Western blot analysis of PEX14 Antibody in HL-60 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with PEX14 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of PEX14 Antibody in HL-60 cell line lysates (35ug/lane)

PEX14 Antibody

CATALOG NUMBER: 63-806

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This PEX14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 343-370 amino acids from the C-terminal region of human PEX14.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
41 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PEX14
Additional Names:
Peroxisomal membrane protein PEX14, PTS1 receptor-docking protein, Peroxin-14, Peroxisomal membrane anchor protein PEX14, PEX14
Protein Accession Number:
O75381
Ncbi Gene Id Number:
5195
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PEX14 belongs to the peroxin 14 family. It is a component of the peroxisomal translocation machinery with PEX13 and PEX17 and interacts with both the PTS1 and PTS2 receptors. PEX14 binds directly to PEX17.Defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK) [MIM:601791] and Zellweger syndrome (ZWS) [MIM:214100].
Background Reference 1:
Neufeld C., et.al., EMBO J. 28:745-754(2009).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-806

List Size:
400 ul

List Price:

$495.00

Datasheet
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