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Western blot analysis of COX6A1 Antibody in MDA-MB435 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human testis tissue reacted with COX6A1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of ZR-75-1 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of COX6A1 using rabbit polyclonal COX6A1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the COX6A1 gene.
Western blot analysis of COX6A1 Antibody in MDA-MB435 cell line lysates (35ug/lane)

COX6A1 Antibody

CATALOG NUMBER: 63-810

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rb
Immunogen:
This COX6A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-78 amino acids from the Central region of human COX6A1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
12 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
COX6A1
Additional Names:
Cytochrome c oxidase subunit 6A1, mitochondrial, Cytochrome c oxidase polypeptide VIa-liver, Cytochrome c oxidase subunit VIA-liver, COX VIa-L, COX6A1, COX6AL
Protein Accession Number:
P12074
Protein Gi Number:
6166030
Ncbi Gene Id Number:
1337
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
Background Reference 1:
Hochstrasser D.F., et.al., Electrophoresis 13:992-1001(1992).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-810

List Size:
400 ul

List Price:

$495.00

Datasheet
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