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Western blot analysis of ATLA2 Antibody in HepG2 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ATLA2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of ATLA2 Antibody in HepG2 cell line lysates (35ug/lane)

ATLA2 Antibody

CATALOG NUMBER: 63-912

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Monkey
Immunogen:
This ATLA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 297-326 amino acids from the Central region of human ATLA2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
66 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ATL2
Additional Names:
Atlastin-2, 365-, ADP-ribosylation factor-like protein 6-interacting protein 2, ARL-6-interacting protein 2, Aip-2, ATL2, ARL6IP2
Protein Accession Number:
Q8NHH9
Protein Gi Number:
147742983
Ncbi Gene Id Number:
64225
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ATLA2 is GTPase tethering membranes through formation of trans-homooligomer and mediating homotypic fusion of endoplasmic reticulum membranes. This protein play a role in endoplasmic reticulum tubular network biogenesis.
Background Reference 1:
Hu,J. Cell 138 (3), 549-561 (2009)
Background Reference 2:
Rismanchi,N. Hum. Mol. Genet. 17 (11), 1591-1604 (2008)
Background Reference 3:
Abel,A. Neurogenetics(4), 239-243 (2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-912

List Size:
400 ul

List Price:

$495.00

Datasheet
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