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Western blot analysis of CSPP1 Antibody in HL-60, Jurkat cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human testis tissue reacted with CSPP1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of CSPP1 Antibody in HL-60, Jurkat cell line lysates (35ug/lane)

CSPP1 Antibody

CATALOG NUMBER: 63-921

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CSPP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-333 amino acids from the N-terminal region of human CSPP1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
146 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CSPP1
Additional Names:
Centrosome and spindle pole-associated protein 1, CSPP1, CSPP
Protein Accession Number:
Q1MSJ5
Protein Gi Number:
313104301
Ncbi Gene Id Number:
79848
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CSPP1 interacts with centrosomes and microtubules and plays a role in cell-cycle progression and spindle organization.
Background Reference 1:
Asiedu, M., et al. Mol. Biol. Cell 20(5):1428-1440(2009) Patzke, S., et al. J. Cell. Physiol. 209(1):199-210(2006) Patzke, S., et al. Oncogene 24(7):1159-1173(2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-921

List Size:
400 ul

List Price:

$495.00

Datasheet
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