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Western Blot at 1:1000 dilution + U-2OS whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of ANKH Antibody in mouse cerebellum tissue lysates (35ug/lane)
ANKH Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:1000 dilution + U-2OS whole cell lysate Lysates/proteins at 20 ug per lane.

ANKH Antibody

CATALOG NUMBER: 64-089

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This ANKH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 464-492 amino acids from the C-terminal region of human ANKH.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
54 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ANKH
Additional Names:
Progressive ankylosis protein homolog, ANK, ANKH, KIAA1581
Protein Accession Number:
Q9HCJ1
Protein Gi Number:
17366849
Ncbi Gene Id Number:
56172
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ANKH is a multipass transmembrane protein that is expressed in joints and other tissues and controls pyrophosphate levels in cultured cells. Progressive ankylosis-mediated control of pyrophosphate levels has been suggested as a possible mechanism regulating tissue calcification and susceptibility to arthritis in higher animals.
Background Reference 1:
Wang, J., et al. J. Rheumatol. 36(6):1265-1272(2009)
Background Reference 2:
Ho, A.M., et al. Science 289(5477):265-270(2000)
Background Reference 3:
Rojas, K., et al. Genomics 62(2):177-183(1999)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

64-089

List Size:
400 ul

List Price:

$495.00

Datasheet
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