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Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized SARS-CoV-2 S1 protein, His Tag at 2 ug/mL (100 uL/well) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 with a linear range of 0.2-3 ng/mL (QC tested).
Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Recombinant

SARS-CoV-2 Spike RBD Antibody, monoclonal, Chimeric

CATALOG NUMBER: 11-587

Clonality:
Recombinant Monoclonal
Tested Applications:
ELISA, WB
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Species Reactivity:
Mouse
Immunogen:
Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 is a chimeric monoclonal antibody combining the constant domains of the human IgG1 molecule with mouse variable regions. The variable region was obtained from a mouse immunized with purified recombinant SARS-CoV-2 Spike S1 Protein.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
This antibody can be paired with other Anti-SARS-CoV-2 Spike S1 antibodies to detect SARS-CoV-2 Spike S1 protein in sandwich ELISA or LFA assay.
Specificity:
This product is a specific antibody against SARS-CoV-2 Spike protein RBD domain. No cross-reactivity is detected with Spike protein RBD domain of other coronaviruses, including SARS-CoV, MERS-CoV, HCoV-229E, HCoV-NL63, HCoV-OC43 and HCoV-HKU1.
Purification:
>95% as determined by SDS-PAGE.
Clonality:
Recombinant Monoclonal
Isotype:
Human IgG1/kappa
physical-state:
Lyophilized
Buffer:
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization.
Concentration:
batch dependent
Storage Conditions:
For long term storage, the product should be stored at lyophilized state at -20°C or lower..Please avoid repeated freeze-thaw cycles.This product is stable after storage at:.-20 to -70°C for 12 months in lyophilized state from date of receipt;.-70°C for 3 months under sterile conditions after reconstitution.
Ncbi Official Symbol:
S
Additional Names:
spike glycoprotein
Ncbi Gene Id Number:
43740568
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

11-587

List Size:
0.1 mg

List Price:

$626.00

Datasheet
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