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Antibody used in WB on Human Muscle at 0.2-1 ug/ml.
Antibody used in WB on Human Muscle at 0.2-1 ug/ml.

ALKBH3 Antibody

CATALOG NUMBER: 27-021

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ALKBH3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ALKBH3 antibody can be used for detection of ALKBH3 by ELISA at 1:1562500. ALKBH3 antibody can be used for detection of ALKBH3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. XBL-10413 - Fetal Skeletal Muscle Tissue Lysate
Predicted Molecular Weight:
33 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store ALKBH3 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
ALKBH3
Additional Names:
ALKBH3, ABH3, DEPC-1, DEPC1, MGC118790, MGC118792, MGC118793, PCA1
Protein Accession Number:
NP_631917
Protein Gi Number:
21040275
Ncbi Gene Id Number:
221120
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 (MIM 610602) and ALKBH3 are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine.The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 (MIM 610602) and ALKBH3 are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 [PubMed 12486230]).
Background Reference 1:
Sundheim, O., (2006) EMBO J. 25 (14), 3389-3397.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-021

List Size:
100 ul

List Price:

$519.00

Datasheet
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