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Antibody used in WB on Human Liver at 0.2-1 ug/ml.
Antibody used in WB on Human Liver at 0.2-1 ug/ml.

SPAG8 Antibody

CATALOG NUMBER: 27-059

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SPAG8.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
SPAG8 antibody can be used for detection of SPAG8 by ELISA at 1:312500. SPAG8 antibody can be used for detection of SPAG8 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. XBL-10409 - Fetal Liver Tissue Lysate
Predicted Molecular Weight:
53 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store SPAG8 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
SPAG8
Additional Names:
SPAG8, BS-84, HSD-1, MGC26201, SMP1, SPAG3, hSMP-1, CT142, CILD28
Protein Accession Number:
NP_758516
Protein Gi Number:
27502373
Ncbi Gene Id Number:
26206
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. SPAG8 is recognized by sperm agglutinating antibodies from an infertile woman. This protein is localized in germ cells of the testis at all stages of spermatogenesis and is localized to the acrosomal region of mature spermatozoa. The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein encoded by this gene is recognized by sperm agglutinating antibodies from an infertile woman. This protein is localized in germ cells of the testis at all stages of spermatogenesis and is localized to the acrosomal region of mature spermatozoa. Alternatively spliced variants that encode different protein isoforms have been described but the full-length sequences of only two have been determined.
Background Reference 1:
Cheng, G.Y., (2007) Asian J. Androl. 9 (1), 23-29.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-059

List Size:
100 ul

List Price:

$519.00

Datasheet
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