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Antibody used in WB on Human Lung at 0.2-1 ug/ml.
Antibody used in WB on Hum. Fetal Liver at 1 ug/ml.
Antibody used in WB on Human Jurkat cells at 1 ug/ml.
Antibody used in WB on Human Lung at 0.2-1 ug/ml.

C2orf3 Antibody

CATALOG NUMBER: 27-135

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human C2orf3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
C2orf3 antibody can be used for detection of C2orf3 by ELISA at 1:12500. C2orf3 antibody can be used for detection of C2orf3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. XBL-10410 - Fetal Lung Tissue Lysate
Predicted Molecular Weight:
89 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store C2orf3 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
GCFC2
Additional Names:
C2orf3, DNABF, GCF, TCF9, C2orf3
Protein Accession Number:
NP_003194
Protein Gi Number:
44890065
Ncbi Gene Id Number:
6936
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The first mRNA transcript isolated for C2orf3 gene was part of an artificial chimera derived from two distinct gene transcripts and a primer used in the cloning process (see Genbank accession M29204). C2orf3 belongs to the GCF family. It is a factor that represses transcription. It binds to the GC-rich sequences (5'-GCGGGGC-3') present in the epidermal growth factor receptor, beta-actin, and calcium-dependent protease promoters.The first mRNA transcript isolated for this gene was part of an artificial chimera derived from two distinct gene transcripts and a primer used in the cloning process (see Genbank accession M29204). A positively charged amino terminus present only in the chimera was determined to bind GC-rich DNA, thus mistakenly thought to identify a transcription factor gene.
Background Reference 1:
Anthoni, H., (2007) Hum. Mol. Genet. 16 (6), 667-677.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-135

List Size:
100 ul

List Price:

$519.00

Datasheet
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