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Antibody used in WB on Human HepG2 at 0.5 ug/ml.
Antibody used in WB on Human HepG2 at 0.5 ug/ml.

ATP7A Antibody

CATALOG NUMBER: 27-946

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ATP7A.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ATP7A antibody can be used for detection of ATP7A by ELISA at 1:1562500. ATP7A antibody can be used for detection of ATP7A by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
30 kDa, 163 kDa, 70 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store ATP7A antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
ATP7A
Additional Names:
ATP7A, MK, MNK, DSMAX, SMAX3
Protein Accession Number:
BAC82353
Protein Gi Number:
34222043
Ncbi Gene Id Number:
538
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The ATP7A gene encodes the Menkes copper-translocating P-type ATPase, a ubiquitous protein that regulates the absorption of copper in the gastrointestinal tract. Inside cells, this protein has a dual function: it delivers copper to cuproenzymes in the Golgi compartment and effluxes excess copper. The trafficking mechanism and catalytic activity combine to facilitate absorption and intercellular transport of copper. Menkes disease, a systemic copper deficiency disorder, is caused by mutations in the ATP7A gene.
Background Reference 1:
Ueta, A., Unpublished.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-946

List Size:
100 ul

List Price:

$473.00

Datasheet
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