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Antibody used in IHC on Human kidney.
Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.
Antibody used in IHC on Human kidney.

TIMELESS Antibody

CATALOG NUMBER: 28-025

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TIMELESS.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
TIMELESS antibody can be used for detection of TIMELESS by ELISA at 1:312500. TIMELESS antibody can be used for detection of TIMELESS by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Tranfected 293T Cell Lysate
Predicted Molecular Weight:
139 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store TIMELESS antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
TIMELESS
Additional Names:
TIMELESS, TIM, TIM1, hTIM
Protein Accession Number:
NP_003911
Protein Gi Number:
222136585
Ncbi Gene Id Number:
8914
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The human Timeless protein interacts with both the circadian clock protein cryptochrome 2 and with the cell cycle checkpoint proteins Chk1 and the ATR-ATRIP complex and plays an important role in the DNA damage checkpoint response. Down-regulation of Timeless in human cells seriously compromises replication and intra-S checkpoints, indicating an intimate connection between the circadian cycle and the DNA damage checkpoints that is in part mediated by the Timeless protein.
Background Reference 1:
Maruyama, K. (2005) Mol. Cell. Biol. 25 (8), 3109-3116.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-025

List Size:
100 ul

List Price:

$519.00

Datasheet
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