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Antibody used in IHC on Human Intestine.
Antibody used in WB on Human HepG2 at 1.25 ug/ml.
Antibody used in IHC on Human Intestine.

TRPM5 Antibody

CATALOG NUMBER: 28-258

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TRPM5.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
TRPM5 antibody can be used for detection of TRPM5 by ELISA at 1:1562500. TRPM5 antibody can be used for detection of TRPM5 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
131 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store TRPM5 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
TRPM5
Additional Names:
TRPM5, LTRPC5, MTR1
Protein Accession Number:
NP_055370
Protein Gi Number:
11225266
Ncbi Gene Id Number:
29850
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TRPM5 is a voltage-modulated Ca (2+)-activated, monovalent cation channel (VCAM) that mediates a transient membrane depolarization and plays a central role in taste transduction. It is activated by arachidonic acid in vitro. It may be involved in perception of bitter, sweet and umami tastes. It may also be involved in sensing semiochemicals.
Background Reference 1:
Liu, D., (2005) J. Biol. Chem. 280 (21), 20691-20699.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-258

List Size:
100 ul

List Price:

$473.00

Datasheet
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