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Antibody used in IHC on Human Intestine.
Antibody used in WB on Human HepG2 at 1.25 ug/ml.
Antibody used in WB on mouse fibroblasts at 1:600.
Antibody used in WB on HepG2 at 2.5 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).
Antibody used in IHC on Human Intestine.

ANXA1 Antibody

CATALOG NUMBER: 28-512

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ANXA1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
ANXA1 antibody can be used for detection of ANXA1 by ELISA at 1:62500. ANXA1 antibody can be used for detection of ANXA1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
38 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store ANXA1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
ANXA1
Additional Names:
ANXA1, ANX1, LPC1
Protein Accession Number:
NP_000691
Protein Gi Number:
4502101
Ncbi Gene Id Number:
301
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ANXA1 encodes a protein that belongs to a family of Ca (2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity.
Background Reference 1:
Ernst, S., et al., (2004) J. Immunol. 172 (12), 7669-7676.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-512

List Size:
100 ul

List Price:

$473.00

Datasheet
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