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Antibody used in IHC on Human kidney.
Antibody used in WB on Human Jurkat 1.25 ug/ml.
Antibody used in WB on Jurkat 2.5 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).
Antibody used in IHC on Human kidney.

SPP1 Antibody

CATALOG NUMBER: 28-547

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SPP1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
SPP1 antibody can be used for detection of SPP1 by ELISA at 1:1562500. SPP1 antibody can be used for detection of SPP1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
33 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store SPP1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
SPP1
Additional Names:
SPP1, OPN, BNSP, BSPI, ETA-1
Protein Accession Number:
NP_000573
Protein Gi Number:
4759166
Ncbi Gene Id Number:
6696
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The SPP1 gene encodes an acidic matrix protein, mainly expressed in mineralized tissues, kidney and atherosclerotic vessels. This protein also contributes to several steps in the process of prostate carcinogenesis and metastasis.
Background Reference 1:
Iwata, M., et al., (2004) Blood 103 (12), 4496-4502.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-547

List Size:
100 ul

List Price:

$473.00

Datasheet
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