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Antibody used in WB on Human Placenta at 0.2-1 ug/ml.
Antibody used in IHC on Human Spleen at 5 ug/ml.
Antibody used in WB on Human Placenta at 0.2-1 ug/ml.

ATF1 Antibody

CATALOG NUMBER: 28-895

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ATF1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ATF1 antibody can be used for detection of ATF1 by ELISA at 1:12500. ATF1 antibody can be used for detection of ATF1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1309 - Human Placenta Lysate
Predicted Molecular Weight:
29 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store ATF1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
ATF1
Additional Names:
ATF1, EWS-ATF1, FUS/ATF-1, TREB36
Protein Accession Number:
NP_005162
Protein Gi Number:
4885073
Ncbi Gene Id Number:
466
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ATF1 binds the cAMP response element (CRE) (consensus: 5'-GTGACGT [AC] [AG]-3'), a sequence present in many viral and cellular promoters. ATF1 binds to the Tax-responsive element (TRE) of HTLV-I. ATF1 mediates PKA-induced stimulation of CRE-reporter genes.
Background Reference 1:
Iwasaki, K., (2007) J. Biol. Chem. 282 (31), 22335-22343.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-895

List Size:
100 ul

List Price:

$519.00

Datasheet
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