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Antibody used in WB on Human HepG2 at 1.25 ug/ml.
Antibody used in WB on Human HepG2 at 1.25 ug/ml.

ZNF496 Antibody

CATALOG NUMBER: 29-113

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ZNF496.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ZNF496 antibody can be used for detection of ZNF496 by ELISA at 1:62500. ZNF496 antibody can be used for detection of ZNF496 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
67 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store ZNF496 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
ZNF496
Additional Names:
ZNF496, NIZP1, ZFP496, ZSCAN49, ZKSCAN17
Protein Accession Number:
NP_116141
Protein Gi Number:
14249386
Ncbi Gene Id Number:
84838
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ZNF496 contains a SCAN box, a KRAB-A domain, and four consensus C2H2-type zinc fingers preceded by a unique finger derivative, referred to herein as the C2HR motif. The C2HR motif functions to mediate protein-protein interaction with the cysteine-rich (C5HCH) domain of NSD1 in a Zn (II)-dependent fashion, and when tethered to RNA polymerase II promoters, represses transcription in an NSD1-dependent manner. ZNF496 contains a novel type of zinc finger motif that functions as a docking site for NSD1 and is more than just a degenerate evolutionary remnant of a C2H2 motif.
Background Reference 1:
Rual, J.F., (2005) Nature 437 (7062), 1173-1178.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

29-113

List Size:
100 ul

List Price:

$473.00

Datasheet
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