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Antibody used in WB on Human kidney at 0.2-1 ug/ml.
Antibody used in WB on Mouse liver at: 1:500 (Lane 1. 60 ug mouse liver extract, Lane 2. 60 ug mouse liver extract, Lane 3. 60 ug mouse liver extract).
Antibody used in WB on Human capan1, HPAF at: 1:1000 (Lane 1. 45ug capan1 cell lysate Lane 2. 45 ug HPAF cell lysate).
Antibody used in IHC on Human Skeletal muscle at 4.0-8.0 ug/ml.
Antibody used in IHC on Human Capan1 at 1:300.
Antibody used in WB on HePG2 at: 1:1000.
Antibody used in WB on Human kidney at 0.2-1 ug/ml.

ACO1 Antibody

CATALOG NUMBER: 29-285

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ACO1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
ACO1 antibody can be used for detection of ACO1 by ELISA at 1:62500. ACO1 antibody can be used for detection of ACO1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. XBL-10408 - Fetal Kidney Tissue Lysate
Predicted Molecular Weight:
98 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store ACO1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
ACO1
Additional Names:
ACO1, IRP1, ACONS, HEL60, IREB1, IREBP, IREBP1
Protein Accession Number:
NP_002188
Protein Gi Number:
8659555
Ncbi Gene Id Number:
48
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ACO1, also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IREs). It plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes.Aconitase 1, also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IREs). IREs are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. The iron-induced binding to the IRE results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degrading transferrin receptor mRNA. Thus, IREB1 plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes.
Background Reference 1:
Clarke, S.L., (2006) EMBO J. 25 (3), 544-553.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

29-285

List Size:
100 ul

List Price:

$519.00

Datasheet
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