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Antibody used in IHC on Human Liver at 4.0-8.0 ug/ml.
Antibody used in WB on Human Jurkat cells at 0.2-1 ug/ml.
Antibody used in IHC on Human Liver at 4.0-8.0 ug/ml.

FECH Antibody

CATALOG NUMBER: 29-655

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human FECH.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
FECH antibody can be used for detection of FECH by ELISA at 1:312500. FECH antibody can be used for detection of FECH by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
47 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store FECH antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
FECH
Additional Names:
FECH, EPP, FCE
Protein Accession Number:
NP_001012533
Protein Gi Number:
60499025
Ncbi Gene Id Number:
2235
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Ferrochelatase is localized to the mitochondrion where it catalyzes the insertion of the ferrous form of iron into protoporphyrin IX in the heme synthesis pathway. Defects in ferrochelatase are associated with protoporphyria.Ferrochelatase is localized to the mitochondrion where it catalyzes the insertion of the ferrous form of iron into protoporphyrin IX in the heme synthesis pathway. Defects in ferrochelatase are associated with protoporphyria. Two transcript variants encoding different isoforms have been found for this gene.Ferrochelatase is localized to the mitochondrion where it catalyzes the insertion of the ferrous form of iron into protoporphyrin IX in the heme synthesis pathway. Defects in ferrochelatase are associated with protoporphyria. Two transcript variants encoding different isoforms have been found for this gene.
Background Reference 1:
Collin, C., (2005) Blood 106 (3), 1098-1104.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

29-655

List Size:
100 ul

List Price:

$519.00

Datasheet
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