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Antibody used in WB on Human Jurkat 5.0 ug/ml.
Antibody used in IHC on Human Kidney at 4.0-8.0 ug/ml.
Antibody used in WB on Human Jurkat 5.0 ug/ml.

ALAS2 Antibody

CATALOG NUMBER: 29-663

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ALAS2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
ALAS2 antibody can be used for detection of ALAS2 by ELISA at 1:62500. ALAS2 antibody can be used for detection of ALAS2 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
57 kDa, 65 kDa, 61 kDa, 63 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store ALAS2 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
ALAS2
Additional Names:
ALAS2, ANH1, ASB, PRO2399, XLSA, ALASE, XLDPP, XLEPP, ALAS-E
Protein Accession Number:
NP_001033058
Protein Gi Number:
189217859
Ncbi Gene Id Number:
212
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ALAS2 specifies an erythroid-specific mitochondrially located enzyme. The protein catalyzes the first step in the heme biosynthetic pathway. Defects in its gene cause X-linked pyridoxine-responsive sideroblastic anemia.The product of this gene specifies an erythroid-specific mitochondrially located enzyme. The encoded protein catalyzes the first step in the heme biosynthetic pathway. Defects in this gene cause X-linked pyridoxine-responsive sideroblastic anemia. Alternatively spliced transcript variants encoding different isoforms have been identified.
Background Reference 1:
Astner, I., (2005) EMBO J. 24 (18), 3166-3177.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

29-663

List Size:
100 ul

List Price:

$473.00

Datasheet
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