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The immunogen is horse cytochrome c. Schematic structure of horse cytochrome c (aa. 1-104) as seen in this image.
The immunogen is horse cytochrome c. Schematic structure of horse cytochrome c (aa. 1-104) as seen in this image.

Cytochrome c Antibody [SJL2-4]

CATALOG NUMBER: XA-1006

Clonality:
Monoclonal
Tested Applications:
WB
Host Species:
Mouse
Species Reactivity:
Goat, Human, Monkey, Mouse, Rat, Sheep
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Goat, Human, Monkey, Mouse, Rat, Sheep
Immunogen:
Cytochrome C (monoclonal SJL2-4) antibody was raised against Horse cytochrome c.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Cytochrome C antibody can be used for detection of horse cytochrome c by western blot. This product also recognizes human, mouse, rat, sheep, goat and monkey apocytochrome c. Method: Cell extracts from 293T cells (2x106) were separated into cytoplasmic and nuclear fraction, resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-cytochrome c antibody SJL2-4 at 1 μg/mL. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG (SBA) and a chemiluminescence detection system.
Purification:
Cytochrome C (monoclonal SJL2-4) antibody was purified to ≥95% in SDS-PAGE.
Clonality:
Monoclonal
Isotype:
IgG2a
Physical State:
Liquid
Buffer:
PBS containing 0.02% sodium azide
Concentration:
batch dependent
Storage Conditions:
Cytochrome c antibody can be stored at 4°C, stable for one year.
Ncbi Gene Id Number:
100053958
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background Reference 1:
Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade: P. Li, et al.; Cell 91, 479 (1997).
Background Reference 2:
Cytochrome c: can't live with it-can't live without it: J.C. Reed; Cell 91, 559 (1997).
Background Reference 3:
Prevention of apoptosis by Bcl-2: release of cytochrome c from mitochondria blocked: J. Yang, et al.; Science 275, 1129 (1997).
Background Reference 4:
Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3: H. Zou, et al.; Cell 90, 405 (1997).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

XA-1006

List Size:
0.1 mg

List Price:

$405.00

Out of stock

Datasheet
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