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The immunogen is recombinant human soluble BAFF, amino acids 83-285. Anti-BAFF (MAb Buffy-1) recognizes the stalk region present in the membrane-bound form of hBAFF. Schematic structure of human soluble BAFF (rec.) (aa 83-285) as seen in this image.
The immunogen is recombinant human soluble BAFF, amino acids 83-285. Anti-BAFF (MAb Buffy-1) recognizes the stalk region present in the membrane-bound form of hBAFF. Schematic structure of human soluble BAFF (rec.) (aa 83-285) as seen in this image.

BAFF Antibody [Buffy-1] (FITC)

CATALOG NUMBER: XA-1011F

Clonality:
Monoclonal
Tested Applications:
Flow
Host Species:
Rat
Species Reactivity:
Human
Conjugate:
FITC
Specifications
Host Species:
Rat
Species Reactivity:
Human
Immunogen:
BAFF (monoclonal Buffy-1) antibody was raised against recombinant human soluble BAFF (aa. 83-285).
Conjugate:
FITC
Tested Applications:
Flow
Application Note:
BAFF antibody detects endogenous protein by Flow Cytometry. Method: 293T cells (5 x 105) expressing surface BAFF vector or Mock transfected, were incubated on ice for 30 min in 50 μl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 4 μg/mL of Buffy-1 antibody. After washing in FACS buffer, FITC-conjugated antibody to rat IgG was added and cells were analyzed by flow cytometry.
Purification:
Anti BAFF (monoclonal Buffy-1) antibody was purified to ≥95% in SDS-PAGE.
Clonality:
Monoclonal
Isotype:
IgG2a
physical-state:
Liquid
Buffer:
PBS containing 0.02% sodium azide
Concentration:
batch dependent
Storage Conditions:
BAFF antibody can be stored at 4°C, stable for one year.
Ncbi Gene Id Number:
10673
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background Reference 1:
BAFF, a novel ligand of the tumor necrosis factor family, stimulates B cell growth: P. Schneider, et al.; J. Exp. Med. 189, 1747 (1999).
Background Reference 2:
BAFF binds to the tumor necrosis factor receptor-like molecule B cell maturation antigen and is important for maintaining the peripheral B cell population: J.S. Thompson, et al.; J. Exp. Med. 192, 129 (2000).
Background Reference 3:
A soluble form of B cell maturation antigen, a receptor for the tumor necrosis factor family member APRIL, inhibits tumor cell growth: P. Rennert, et al.; J. Exp. Med. 192, 1677 (2000).

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

XA-1011F

List Size:
100 tests

List Price:

$577.00

Datasheet
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