BAFF Antibody [Buffy-2]

Application Note:

BAFF antibody can be used to recognize membrane-bound and soluble human and mouse (weak) BAFF by Flow Cytometry, Immunohistochemistry (frozen sections, paraffin sections) and Western Blot. Method: HEK 293T cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of full length hBAFF (thick line). Cells were incubated on ice for 30min in 50 μl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1 μg/mL of MAb to BAFF (Buffy-2) (FITC). After washing, cells were analyzed by flow cytometry. Method: Tissues are in paraffin sections. Sections are de-waxed and rehydrated, then cooked. Sections are stained in wet boxes, rehydrated and finally prepared as coverslip slides using Eukitt solution. Please inquire for complete protocol..