NALP1 Antibody [Nalpy1-4]

Application Note:

NALP1 can be used to recognize the pyrin domain (PYD) of human NALP1 by Immunocytochemistry (1:1,000), Immunohistochemistry (frozen sections, paraffin sections (microwave treatment)), Immunoprecipitation, and Western Blot. Method: Assembly of the inflammasome was induced by shifting the temperature to 30°C after hypotonic lysis. THP-1 cell extracts were immunoprecipitated with PAb to Asc (AL177) and run on SDS-PAGE. NALP1 was detected by MAb to NALP1 (Nalpy1-4) at 1:1'000 dilution. Anti-mouse IgG coupled horse radish peroxidase was used at 1:5,000 dilution for ECL detection. For more information about the "time course assembly of inflammasome" method see F. Martinon; Mol. Cell. 10, 417 (2002). Method: 293T cells transfected with full length human NALP1 were fixed with 4% paraformaldehyde and stained with 1 μg/mL MAb to NALP1 (Nalpy1-4) and then Alexa 488 (1:300 dilution). This product is for research use only.