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42-014 (0.5ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-014 (3.8ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-014 (1ug/ml) staining of Mouse (A) and Rat (B) Brain (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-014 (0.5ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

STK39 Antibody

CATALOG NUMBER: 42-014

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-SQEKSRRVKEENPE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 65kDa band observed in Human Brain (Cerebellum) lysates and in lysates of cell line Jurkat (calculated MW of 59.5kDa according to NP_037365.2). Approx 75kDa band observed in Mouse and Rat Brain lysates (calculated MW of 60.3kDa according to Mouse NP_058562.1). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:In paraffin embedded Human Cerebral Cortex shows textured staining in the neurons. Recommended concentration, 3-5ug/ml.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
STK39
Additional Names:
STK39, serine threonine kinase 39 (STE20/SPS1 homolog, yeast), DCHT, DKFZp686K05124, PASK, SPAK, Ste20-like protein kinase, proline-alanine-rich STE20-related kinase, small intestine SPAK-like kinase
Protein Accession Number:
NP_037365.2
Protein Gi Number:
115430252
Ncbi Gene Id Number:
27347
Background Reference 1:
Ramoz N, Cai G, Reichert JG, Silverman JM, Buxbaum JD. An analysis of candidate autism loci on chromosome 2q24-q33: evidence for association to the STK39 gene. Am J Med Genet B Neuropsychiatr Genet. 2008 Oct 5;147B(7):1152-8

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-014

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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