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45-154 (4ug/ml) staining of paraffin embedded Mouse Thymus. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
45-154 (4ug/ml) staining of paraffin embedded Mouse Thymus. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

STAT3 Antibody

CATALOG NUMBER: 45-154

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, IF, IHC
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Homology:
Expected Species Reactivity based on sequence homology: Human, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: DMELTSECATSPM
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Immunohistochemistry:In paraffin embedded Mouse Thymus shows preferential staining of the Medulla over the Cortex. Recommended concentration: 4-6ug/ml.Immunofluorescence:Strong expression of the protein seen in the cytoplasm of HeLa and A431 cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
Specificity:
This antibody is expected to recognise isoforms 1 and 2 (as represented by NP_644805.1 and NP_003141.2 respectively)
Positive Control 1:
Cat. No. 1202 - A431 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
STAT3
Additional Names:
STAT3, signal transducer and activator of transcription 3 (acute-phase response factor), APRF, DNA-binding protein APRF, acute-phase response factor, FLJ20882, MGC16063, HIES
Protein Accession Number:
NP_003141.2,NP_644805.1
Protein Gi Number:
21618338
Ncbi Gene Id Number:
6774
Background Reference 1:
Bromberg JF, Wrzeszczynska MH, Devgan G, Zhao Y, Pestell RG, Albanese C, Darnell JE Jr. Stat3 as an oncogene. Cell. 1999 Aug 6;98(3):295-303. Erratum in: Cell 1999 Oct 15;99(2):239.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-154

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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