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42-081 (3.75ug/ml) staining of paraffin embedded Mouse Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-081 (3.75ug/ml) staining of paraffin embedded Mouse Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Mcpt4 Antibody

CATALOG NUMBER: 42-081

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Immunogen:
The immunogen for this antibody is: C-KLQKKAKETPSVN
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave bands at approx. 40kDa and 15kDa in Mouse Skeletal Muscle lysates after 0.01ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 27.2kDa according to NP_034909.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).Immunohistochemistry:Paraffin embedded Mouse Skeletal Muscle. Recommended concentration: 3.75ug/ml.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
Mcpt4
Additional Names:
Mcpt4, mast cell protease 4, MMCP-4, MMCP-4A, MMCP-4B, Mcp-4, myonase
Protein Accession Number:
NP_034909.2
Protein Gi Number:
114205406
Ncbi Gene Id Number:
17227(mouse);
Background Reference 1:
Kitaura-Inenaga K, Hara M, Higuchi K, Yamamoto K, Yamaki A, Ono K, Nakano A, Kinoshita M, Sasayama S, Matsumori A. Gene expression of cardiac mast cell chymase and tryptase in a murine model of heart failure caused by viral myocarditis. Circ. J. 2003 Oct 67 (10): 881-4.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-081

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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