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42-092 (50ug/ml) staining of islets in Mouse Pancreas. Citrate antigen retrieval. Detected by immunofluorescence.
42-092 (3.8ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-092 (50ug/ml) staining of islets in Mouse Pancreas. Citrate antigen retrieval. Detected by immunofluorescence.

UNC5C Antibody

CATALOG NUMBER: 42-092

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: NCTVSEEPTGID
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments in Human and Mouse Brain lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml).Immunohistochemistry:In paraffin embedded Human Cerebral Cortex shows staining of some neuronal bodies plus intense axonal staining. Recommended concentration: 3-5ug/ml.Immunofluorescence: Staining of paraffin embedded endocrine islets (red) in adult Mouse Pancreas. Nuclear staining in blue and ductal cell staining in green. Data obtained from a customer.
Positive Control 1:
Cat. No. 1414 - Mouse Pancreas Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
UNC5C
Additional Names:
UNC5C, unc-5 homolog C (C. elegans), UNC5H3, homolog of C. elegans transmembrane receptor Unc5, unc5 (C.elegans homolog) c, unc5C
Protein Accession Number:
NP_003719.2
Protein Gi Number:
16933525
Ncbi Gene Id Number:
8633
Background Reference 1:
Shin SK, Nagasaka T, Jung BH, Matsubara N, Kim WH, Carethers JM, Boland CR, Goel A. Epigenetic and genetic alterations in Netrin-1 receptors UNC5C and DCC in human colon cancer. Gastroenterology 2007 Dec 133 (6): 1849-57.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-092

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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