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42-188 (1ug/ml) staining of Mouse Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-188 (1ug/ml) staining of Mouse Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

SUMF1 Antibody

CATALOG NUMBER: 42-188

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-ETLNPKGPPSGKDR
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 37-40kDa band observed in Mouse Pancreas, Mouse Eye and Human Kidney lysates (calculated MW of 40.6kDa according to Human NP_877437.2 and 40.6kDa according to Mouse NP_666049.2). Recommended concentration: 0.5-2ug/ml.
Positive Control 1:
Cat. No. 1414 - Mouse Pancreas Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SUMF1
Additional Names:
SUMF1, sulfatase modifying factor 1, AAPA3037, FGE, MGC131853, MGC150436, C-alpha-formylglycine-generating enzyme, OTTHUMP00000115300
Protein Accession Number:
NP_877437.2
Protein Gi Number:
38202250
Ncbi Gene Id Number:
285362
Background Reference 1:
Oshikawa M, Usami R, Kato S. Characterization of the arylsulfatase I (ARSI) gene preferentially expressed in the human retinal pigment epithelium cell line ARPE-19. Molecular vision 2009 15 : 482-94.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-188

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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