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ALS Antibody

CATALOG NUMBER: 42-291

Clonality:
Polyclonal
Tested Applications:
ELISA
Host Species:
Goat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Rat, Dog, Cow, Pig
Immunogen:
The immunogen for this antibody is: C-GLDLRDLSEAH
Conjugate:
Unconjugated
Tested Applications:
ELISA
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 45kDa band in lysates of cell line HepG2 after 2ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 66.0kDa according to NP_004961.1. The 45kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
IGFALS
Additional Names:
IGFALS, insulin-like growth factor binding protein, acid labile subunit, ALS, INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN COMPLEX ACID LABILE CHAIN
Protein Accession Number:
NP_004961.1
Protein Gi Number:
4826772
Ncbi Gene Id Number:
3483
Background Reference 1:
Hosgood HD, Menashe I, Shen M, Yeager M, Yuenger J, Rajaraman P, He X, Chatterjee N, Caporaso NE, Zhu Y, Chanock SJ, Zheng T, Lan Q. Pathway-based evaluation of 380 candidate genes and lung cancer susceptibility suggests the importance of the cell cycle pathway. Carcinogenesis 2008 Oct 29 (10): 1938-43.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-291

List Size:
0.1 mg

List Price:

$391.00

Datasheet
Shipping Info

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