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42-339 (0.5ug/ml) staining of paraffin embedded Mouse Brain stem in sagittal (left) and coronal (right) sections of Raphe nuclei. Detection Alexa 594. Data obtained from Prof. D Crane, Griffith Univeristy, Brisbane, Australia
42-339 (0.5ug/ml) staining of paraffin embedded Mouse Brain stem in sagittal (left) and coronal (right) sections of Raphe nuclei. Detection Alexa 594. Data obtained from Prof. D Crane, Griffith Univeristy, Brisbane, Australia

Tph2 Antibody

CATALOG NUMBER: 42-339

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Immunogen:
The immunogen for this antibody is: C-SLTQNKAIKSEDK
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 85kDa band in Mouse fetal Brain lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 55.9kDa according to NP_775567.2. The 85kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:In paraffin embedded Mouse Brain stem shows staining of Raphe nucleii serotonergic neurons consistent with observation by different antibodies. Recommended concentration, 0.5-2ug/ml.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
Tph2
Additional Names:
Tph2, tryptophan hydroxylase 2, AU043594, MGC159133, Ntph, OTTMUSP00000022885, neuronal tryptophan hydroxylase, tryptophan 5-hydroxylase 2, tryptophan 5-monooxygenase 2
Protein Accession Number:
NP_775567.2
Protein Gi Number:
148540044
Ncbi Gene Id Number:
216343(mouse);

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-339

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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