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45-219 (1ug/ml) staining of Human (A) and (0.5ug/ml) of Mouse (B) and Rat (C) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-219 (2ug/ml) staining of paraffin embedded Mouse Brain. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.<strong>This data is from a previous batch, not on sale.</strong>
45-219 (5ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with Tris/EDTA buffer pH 9, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
45-219 (5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
45-219 (5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
45-219 (5ug/ml) staining of paraffin embedded Human Lung. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining. <strong>This data is from a previous batch, not on sale.</strong>
45-219 Negative Control showing staining of paraffin embedded Human Lung, with no primary antibody. <strong>This data is from a previous batch, not on sale.</strong>
45-219 (1ug/ml) staining of Human (A) and (0.5ug/ml) of Mouse (B) and Rat (C) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

AIF1 Antibody

CATALOG NUMBER: 45-219

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Pig
Immunogen:
The immunogen for this antibody is: C-TGPPAKKAISELP
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 17kDa band observed in Human Brain (Frontal Cortex) and Rat Brain lysates (calculated MW of 16.7kDa according to Human NP_001614.3 and 16.8kDa according to Rat NP_058892.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.This antibody can also be purchased as directly conjugated to HRP. Please specify when you order.
Specificity:
This antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3).
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
AIF1
Additional Names:
AIF1, IBLY409840A1, allograft inflammatory factor 1, AIF-1, IRT-1, interferon gamma responsive transcript, ionized calcium-binding adapter molecule, Daintain, DADB-70P7.8
Protein Accession Number:
NP_116573.1,NP_001614.3
Protein Gi Number:
14574566
Ncbi Gene Id Number:
199
Background Reference 1:
Autieri MV, Agrawal N. IRT-1, a novel interferon-gamma-responsive transcript encoding a growth-suppressing basic leucine zipper protein. J Biol Chem. 1998 Jun 12;273(24):14731-7.
Background Reference 2:
Clavaguera F, Bolmont T, Crowther RA, Abramowski D, Frank S, Probst A, Fraser G, Stalder AK, Beibel M, Staufenbiel M, Jucker M, Goedert M, Tolnay M. Transmission and spreading of tauopathy in transgenic mouse brain. Nat Cell Biol. 2009 Jul;11(7):909-13.
Background Reference 3:
Kalm M, Lannering B, Björk-Eriksson T, Blomgren K. Irradiation-induced loss of microglia in the young brain. J Neuroimmunol. 2009 Jan 3;206(1-2):70-
Background Reference 4:
Hirko AC, Dallasen R, Jomura S, Xu Y. Modulation of inflammatory responses after global ischemia by transplanted umbilical cord matrix stem cells. Stem Cells. 2008 Nov;26(11):2893-901.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-219

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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