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42-388 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI
42-388 Flow cytometric analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton, showing a low level of staining. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Un
42-388 (0.3ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-388 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI

Sirt3 Antibody

CATALOG NUMBER: 42-388

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, IF, WB
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Homology:
Expected Species Reactivity based on sequence homology: Human, Rat, Pig
Immunogen:
The immunogen for this antibody is: C-RASGIPASKLVEAH
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 30kDa band observed in Mouse Heart lysates (calculated MW of 28.8kDa according to NP_071878.2). An additional band of unknown identity was also consistently observed at 15kDa. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat Kidney for this particular batch.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of NIH3T3 cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of NIH3T3 cells. Recommended concentration: 10ug/ml.

Specificity:
This antibody is expected to recognize both reported isoforms (NP_071878.2; NP_001171275.1). Reported variants represent identical proteins: NP_071878.2; NP_001120823.1
Positive Control 1:
Cat. No. 1401 - Mouse Heart Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
Sirt3
Additional Names:
Sirt3, sirtuin 3, sirtuin 3 (silent mating type information regulation 2, homolog) 3, 2310003L23Rik, AI848213, Sir2l3, NAD-dependent deacetylase sirtuin-3, OTTMUSP00000027441, OTTMUSP00000027442, OTTMUSP00000027444, OTTMUSP00000027445, SIR2-like protein 3, SIRT3L mitochondrial, mSIR2L3, mitochondrial protein lysine deacetylase, silent mating type information regulation 2, (S.cerevisiae, homolog)-like 3
Protein Accession Number:
NP_071878.2,NP_001171275.1
Protein Gi Number:
188035865
Ncbi Gene Id Number:
23410

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-388

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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