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42-428 (0.05ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-428 (3.8ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-428 (0.05ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

SIRT1 Antibody

CATALOG NUMBER: 42-428

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse
Immunogen:
The immunogen for this antibody is: C-EKPQEVQTSRNVES
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 120kDa band observed in lysates of cell line Jurkat (calculated MW of 81.7kDa according to NP_036370.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Cheng et al, Proc Natl Acad Sci U S A. 2003 Sep 16;100(19):10794-9.; PMID: 12960381). Recommended concentration: 0.05-0.2ug/ml.Immunohistochemistry:In paraffin embedded Human Skin shows perinuclear staining in select keratinocytes. Recommended concentration, 3-5ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_036370.2; NP_001135970.1).
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SIRT1
Additional Names:
SIRT1, sirtuin 1, SIR2L1, NAD-dependent deacetylase sirtuin-1, OTTHUMP00000198111, OTTHUMP00000198112, SIR2-like protein 1, SIR2alpha, hSIR2, hSIRT1, sir2-like 1, sirtuin type 1
Protein Accession Number:
NP_036370.2,NP_001135970.1
Protein Gi Number:
7657575
Ncbi Gene Id Number:
23411

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-428

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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