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42-458 (5ug/ml) staining of paraffin embedded Human Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-458 (5ug/ml) staining of paraffin embedded Human Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

TSPYL2 Antibody

CATALOG NUMBER: 42-458

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-PNGWANPGKRGKTG
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments in Human Testis lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunohistochemistry:In paraffin embedded Human Testis shows nucleolar staining in spermatocytes. Recommended concentration, 5-10ug/ml.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TSPYL2
Additional Names:
TSPYL2, TSPY-like 2, RP1-290F12.2, CDA1, CINAP, CTCL, DENTT, HRIHFB2216, NP79, SE204, TSPX, CASK-interacting nucleosome assembly protein, CTCL tumor antigen se20-4, CTCL-associated antigen se20-4, TSPY-like protein 2, cell division autoantigen 1, cutaneous T-cell lymphoma-associated antigen se20-4, cutaneous T-cell lymphoma-associated tumor antigen se20-4, differentially expressed nucleolar TGF-beta1 target, differentially-expressed nucleolar TGF-beta1 target protein, nuclear protein of 79 kDa, testis-specific Y-encoded-like protein 2, testis-specific protein Y encoded-like 2
Protein Accession Number:
NP_071400.1
Protein Gi Number:
11545835
Ncbi Gene Id Number:
64061

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-458

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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